Populus nigra L. bud absolute: a case study for a strategy of analysis of natural complex substances.
Identifieur interne : 002532 ( Main/Exploration ); précédent : 002531; suivant : 002533Populus nigra L. bud absolute: a case study for a strategy of analysis of natural complex substances.
Auteurs : Patrizia Rubiolo [Italie] ; Cristina Casetta ; Cecilia Cagliero ; Hugues Brevard ; Barbara Sgorbini ; Carlo BicchiSource :
- Analytical and bioanalytical chemistry [ 1618-2650 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Extraits de plantes, Flavonoïdes, Phénols.
- composition chimique : Populus.
- Chromatographie en phase liquide à haute performance, Chromatographie gazeuse-spectrométrie de masse.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Flavonoids, Phenols, Plant Extracts.
- chemistry : Populus.
- Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry.
Abstract
The new European regulations (e.g., REACH) require that Natural Complex Substances such as essential oils, absolutes, concretes, and resinoids are registered. This need implies that the chemical composition of these complex mixtures is characterized as exhaustively as possible in view of defining their toxicological risk. This study proposes an analysis strategy to be applied to the chemical characterization of poplar absolute as an example of Natural Complex Substances of vegetable origin. In the first part, the proposed strategy is described, and the advantages and the limitations related to the combination of conventional analytical techniques such as gas chromatography (GC) without and with sample derivatization and high-performance liquid chromatography (HPLC) are critically discussed. In the second part, the qualitative data obtained with GC and HPLC analysis of poplar bud absolute confirm the sample complexity which mainly consists of phenolic components. Fourteen compounds (i.e., phenolic acids, phenylpropanoids, and flavonoids) were then chosen as markers representative of the main classes of components characterizing poplar bud absolute. The marker quantitation carried out by GC-SIM-MS and HPLC-PDA analyses gives similar results confirming the reliability of both techniques. These results demonstrate that conventional analytical techniques can positively and effectively contribute to the study of the the composition of Natural Complex Substances, i.e., matrices for which highly effective separation is necessary, consisting mainly of isomers or homologous components. The combination of GC and HPLC techniques is ever more necessary for routine quality control when conventional instrumentations are used.
DOI: 10.1007/s00216-012-6537-y
PubMed: 23180080
Affiliations:
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sort="Cagliero, Cecilia" uniqKey="Cagliero C" first="Cecilia" last="Cagliero">Cecilia Cagliero</name></author><author><name sortKey="Brevard, Hugues" sort="Brevard, Hugues" uniqKey="Brevard H" first="Hugues" last="Brevard">Hugues Brevard</name></author><author><name sortKey="Sgorbini, Barbara" sort="Sgorbini, Barbara" uniqKey="Sgorbini B" first="Barbara" last="Sgorbini">Barbara Sgorbini</name></author><author><name sortKey="Bicchi, Carlo" sort="Bicchi, Carlo" uniqKey="Bicchi C" first="Carlo" last="Bicchi">Carlo Bicchi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23180080</idno><idno type="pmid">23180080</idno><idno type="doi">10.1007/s00216-012-6537-y</idno><idno type="wicri:Area/Main/Corpus">002792</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002792</idno><idno type="wicri:Area/Main/Curation">002792</idno><idno type="wicri:explorRef" 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(MeSH)</term><term>Flavonoids (analysis)</term><term>Gas Chromatography-Mass Spectrometry (MeSH)</term><term>Phenols (analysis)</term><term>Plant Extracts (analysis)</term><term>Populus (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Chromatographie gazeuse-spectrométrie de masse (MeSH)</term><term>Extraits de plantes (analyse)</term><term>Flavonoïdes (analyse)</term><term>Phénols (analyse)</term><term>Populus (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Flavonoids</term><term>Phenols</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Extraits de plantes</term><term>Flavonoïdes</term><term>Phénols</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Gas Chromatography-Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Chromatographie gazeuse-spectrométrie de masse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The new European regulations (e.g., REACH) require that Natural Complex Substances such as essential oils, absolutes, concretes, and resinoids are registered. This need implies that the chemical composition of these complex mixtures is characterized as exhaustively as possible in view of defining their toxicological risk. This study proposes an analysis strategy to be applied to the chemical characterization of poplar absolute as an example of Natural Complex Substances of vegetable origin. In the first part, the proposed strategy is described, and the advantages and the limitations related to the combination of conventional analytical techniques such as gas chromatography (GC) without and with sample derivatization and high-performance liquid chromatography (HPLC) are critically discussed. In the second part, the qualitative data obtained with GC and HPLC analysis of poplar bud absolute confirm the sample complexity which mainly consists of phenolic components. Fourteen compounds (i.e., phenolic acids, phenylpropanoids, and flavonoids) were then chosen as markers representative of the main classes of components characterizing poplar bud absolute. The marker quantitation carried out by GC-SIM-MS and HPLC-PDA analyses gives similar results confirming the reliability of both techniques. These results demonstrate that conventional analytical techniques can positively and effectively contribute to the study of the the composition of Natural Complex Substances, i.e., matrices for which highly effective separation is necessary, consisting mainly of isomers or homologous components. The combination of GC and HPLC techniques is ever more necessary for routine quality control when conventional instrumentations are used.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23180080</PMID><DateCompleted><Year>2013</Year><Month>06</Month><Day>24</Day></DateCompleted><DateRevised><Year>2016</Year><Month>05</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-2650</ISSN><JournalIssue CitedMedium="Internet"><Volume>405</Volume><Issue>4</Issue><PubDate><Year>2013</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Analytical and bioanalytical chemistry</Title><ISOAbbreviation>Anal Bioanal Chem</ISOAbbreviation></Journal><ArticleTitle>Populus nigra L. bud absolute: a case study for a strategy of analysis of natural complex substances.</ArticleTitle><Pagination><MedlinePgn>1223-35</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00216-012-6537-y</ELocationID><Abstract><AbstractText>The new European regulations (e.g., REACH) require that Natural Complex Substances such as essential oils, absolutes, concretes, and resinoids are registered. This need implies that the chemical composition of these complex mixtures is characterized as exhaustively as possible in view of defining their toxicological risk. This study proposes an analysis strategy to be applied to the chemical characterization of poplar absolute as an example of Natural Complex Substances of vegetable origin. In the first part, the proposed strategy is described, and the advantages and the limitations related to the combination of conventional analytical techniques such as gas chromatography (GC) without and with sample derivatization and high-performance liquid chromatography (HPLC) are critically discussed. In the second part, the qualitative data obtained with GC and HPLC analysis of poplar bud absolute confirm the sample complexity which mainly consists of phenolic components. Fourteen compounds (i.e., phenolic acids, phenylpropanoids, and flavonoids) were then chosen as markers representative of the main classes of components characterizing poplar bud absolute. The marker quantitation carried out by GC-SIM-MS and HPLC-PDA analyses gives similar results confirming the reliability of both techniques. These results demonstrate that conventional analytical techniques can positively and effectively contribute to the study of the the composition of Natural Complex Substances, i.e., matrices for which highly effective separation is necessary, consisting mainly of isomers or homologous components. 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